|Test||CHROMOSOME ANALYSIS, CHORIONIC VILLUS|
|Methodology||Includes 2 - banded karyotypes, analysis of 20 or more metaphases whenever possible, and other techniques when required.|
|Description|| This test is useful for the prenatal diagnosis of chromosome abnormalities (trisomies, deletion, translocations, etc.) at about 9 - 12 weeks of gestation.
NOTE: This test, when ordered on Medicare patients, is subject to the CMS Medicare Coverage Issues Manual (MCIM) Diagnostic Services policy "Cytogenetic Studies". Please verify that the diagnosis code (ICD-9) you have chosen demonstrates medical necessity for the test as documented in the physician's patient record. The CMS web site or the NorDx Limited Coverage Documentation Guide are available to assist you with this verification. A properly executed Advance Beneficiary Notice (ABN) must be submitted with the specimen if medical necessity is not demonstrated by the ICD-9 code chosen.
|Specimen||Please provide a reason for referral with each specimen by completing a "Cytogenetics/AFP Congenital Disorders Request Form".
Obtain 20 mg (minimum: 5 mg) of chorionic villus specimen (CVS) by the transabdominal or transcervical method. Transfer the CVS to a Petri dish containing transport medium. Using a stereo microscope and sterile forceps, assess the quality and quantity of the villi and remove any blood clots. Transfer the CVS by using sterile technique to one or two 15-mL centrifuge tube(s) with 15 mL of transport medium. (Petri dishes and transport medium are supplied by NorDx.) Label the centrifuge tube(s) with patient's name and laboratory control number. Send specimen refrigerated. SPECIMEN CANNOT BE FROZEN.
|Reference Values||46,XX or 46,XY. No apparent chromosome abnormality.|
|Analytic Time||6 - 12 Days|
|Day(s) Test Set Up||Monday through Sunday|
88235 Amniotic culture
88267 Chorionic villus
88280 Additional karyotypes, each study
88291 Interpretation and report