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STCUL
CULTURE, BACTERIA, STOOL

Test Code STCUL 
Test CULTURE, BACTERIA, STOOL 
Methodology Conventional culture with enzyme immunoassay for shigatoxin. 
Description Separate charges are applied for the culture and the
immunoassay. Significant isolates reflex to organism identification and antibiotic susceptibility test. Additional charges apply. Useful for the detection of Salmonella, Shigella, Campylobacter, Aeromonas, Pleisiomonas, E. coli O157 and other shiga toxin producing E.coli strains. 
Specimen SUBMIT ONLY 1 OF THE FOLLOWING SPECIMENS:

PREFERRED
PRESERVED STOOL
Submit specimen in Para-Pak® C&S (Cary-Blair) transport medium collected as follows:
1. Collect stool specimen. Do not contaminate with urine or water.
2. Using spoon attached to cap of container select portions of specimen containing pus, blood, or mucous.
3. Add a sufficient amount of specimen to transport media to bring liquid level up to “fill to here” line. Do not fill above line indicated on containers.
4. Agitate specimen with spoon along side of container, tighten cap, and shake firmly to insure that specimen is adequately mixed (solution should appear homogeneous).
5. Label container with patient’s name (first and last), date of birth or medical record number, date and time of collection, collector’s initials and SPECIMEN SOURCE.
6. Place container(s) in a Ziplock® bag.
7. Maintain sterility and forward promptly. The Para-Pak® transport system preserves enteric pathogens for 96 hours at ambient temperature.
NOTE:
1. For pathogens other than those listed above (eg, vibrio, yersinia), specify pathogens suspected.
2. SPECIMEN SOURCE IS REQUIRED ON REQUEST FORM FOR PROCESSING.

ALTERNATE:
UNPRESERVED STOOL
Specimen must arrive within 2 hours of collection.
1. Submit 10 g of fresh stool in a screw-capped, sterile container. Do not contaminate with urine or water. No preservative.
2. Label container with patient’s name (first and last), date of birth or medical record number, date and time of collection, collector’s initials and specimen source.
3. Place container in a Ziplock® bag.
4. Maintain sterility and forward promptly. For optimum detection of pathogens transport time should be less than two hours. Recovery of some pathogens (especially Shigella species) is reduced with longer transport times.
5. Send specimen refrigerated.
NOTE:
1. For pathogens other than those listed above (eg, vibrio, yersinia), specify pathogens suspected.
2. SPECIMEN SOURCE IS REQUIRED ON REQUEST FORM FOR PROCESSING. 

MMC Specimen Specimens collected from hospitalized patients more than 3 days after admission seldom contain Salmonella, Shigella, Campylobacter or E. coli O157. These specimens are not routinely processed for culture. 
Reference Values NEGATIVE for Salmonella, Shigella, Campylobacter, Aeromonas, Pleisiomonas, E. coli 0157. Shigatoxin not detected. 
Analytic Time 4 Days 
Day(s) Test Set Up Monday through Sunday 
CPT Code(s) 87045  Stool culture for bacteria
87046 x 3  Culture, additional pathogens
87077  Bacterial identification, if indicated
87149 x5  CRE Confirmation, if indicated
87181  Antibiotic susceptibility (gradient strip), per antibiotic tested, if indicated
87184  Antibiotic susceptibility (disc method), if indicated
87186  Antibiotic susceptibility (MIC), if indicated
87427  Shiga toxin assay