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UFRAC
UNIFRACTIONATED HEPARIN LEVEL

Test Code UFRAC 
Test UNIFRACTIONATED HEPARIN LEVEL 
Methodology Automated Colorimetric 
Description Useful for determination of heparin (unfractionated) concentrations in plasma 
Specimen Collect specimen as follows:
1. Draw blood in light blue-top (3.2% sodium citrate) tube(s). Separate plasma from cells within 1 hour of collection in a centrifuge at 2,500 rpm for 15 minutes or 3,000 rpm for 10 minutes.
2. Using a plastic transfer pipette (do not use a glass pipette) decant the plasma to a screw-capped, plastic (polypropylene) vial. (Glass vials will not be accepted.)
3. Obtain a platelet count on the platelet-poor plasma. If the platelet count is > 10,000, respin the specimen and repeat steps 2 and 3 until the platelet count is < 10,000.
4. Divide the specimen into as many 0.5 - 1.0 mL aliquots as possible and cap the vial(s) tightly.
5. Freeze specimen(s) immediately at <= -40 C.
6. Send specimen(s) FROZEN on dry ice.
NOTE:
1. TYPE OF HEPARIN USED IS REQUIRED ON REQUEST FORM FOR PROCESSING (i.e., unfractionated, Lovenox, Fragmin, or Tinzaparin)
2. SPECIMENS WILL BE REJECTED IF UNDERFILLED
(< 90%), CLOTTED, HIGH HEMATOCRIT (> 55%), OR GROSSLY HEMOLYZED.
3. Follow special procedure for drawing specimen from an A-line. 
Reference Values The therapeutic range (High Intensity) :0.3 - 0.7 IU/mL (drawn at least 6 hours after initiation and/or 6 hours after rate change). 
Analytic Time 1 Day 
Day(s) Test Set Up Set up Monday through Friday between 8 AM and 3 PM 
CPT Code(s) 85520