Test Code ESTF/ESTF-M 
Methodology Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) 
Description This test allows the simultaneous high-sensitivity determination of serum estrone and estradiol levels. It is useful in situations requiring either higher sensitivity estradiol measurement, or estrone measurement, or both. This includes the following:

-As part of the diagnosis and workup of precocious and delayed puberty in females, and, to a lesser degree, males

-As part of the diagnosis and workup of suspected disorders of sex steroid metabolism, eg, aromatase deficiency and 17 alpha-hydroxylase deficiency

-As an adjunct to clinical assessment, imaging studies, and bone mineral density measurement in the fracture risk assessment of postmenopausal women, and, to a lesser degree, older men

-Monitoring low-dose female hormone replacement therapy in postmenopausal women

-Monitoring antiestrogen therapy (eg, aromatase inhibitor therapy)

Useful in all applications that require moderately sensitive measurement of estradiol including:

-Evaluation of hypogonadism and oligo-amenorrhea in females

-Assessing ovarian status, including follicle development, for assisted reproduction protocols (eg, in vitro fertilization)

In conjunction with luteinizing hormone measurements, monitoring of estrogen replacement therapy in hypogonadal premenopausal women

Evaluation of feminization, including gynecomastia, in males

Diagnosis of estrogen-producing neoplasms in males, and, to a lesser degree, females 

Specimen Draw blood in a Plain Red Top tube. Spin down and send 1.2 mL of serum refrigerated in a screw-capped, plastic vial
Serum must be separated from gel within 24 hours of draw. 
Reference Values Estrone Reference Ranges 
Analytic Time 2 Days 
Day(s) Test Set Up Monday through Saturday; 1 p.m 
CPT Code(s) 82679