|Test||HIV 1/2 ANTIBODIES + HIV ANTIGEN, (REFLEX HIV-1 RNA VIRAL LOAD)|
|Methodology||HIV 1/2 Antibodies + HIV Antigen: Chemiluminescent Microparticle Immunoassay (CMIA),
HIV-1 RNA VIRAL LOAD (If indicated): COBAS Ampliprep/COBAS Taqman HIV-1 Test, Quantitative Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)
|Description||Useful for the simultaneous qualitative detection of antibodes to Human Immunodeficiency Virus (HIV), Type 1 (HIV-1 group M and group O) and/or Type 2, and of HIV p24 antigen.
NOTE: This test should not be ordered on children less than 2 years of age. The recommended test for this population is "HIV-1 RNA VIRAL LOAD (HIVVL)
NOTE: Reactive (positive) specimens reflex to the Multispot HIV-1/HIV-2 Differentiation Test at an additional charge. Negative or undifferentiated HIV1 + HIV2 Ab differentiation (HIVSP), reflexes to HIV RNA QN PCR (HIVVL).
NOTE: This test, when ordered on Medicare patients, is subject to the National Coverage Determination (NCD) policy " Human Immunodeficiency Virus (HIV) Testing (Diagnosis)". Please verify that the diagnosis code (ICD-9) you have chosen demonstrates medical necessity for the test as documented in the physician's patient record. The CMS web site or the NorDx Medical Necessity Reference Guide are available to assist you with this verification. A properly executed Advance Beneficiary Notice (ABN) must be submitted with the specimen if medical necessity is not demonstrated by the ICD-9 code chosen or if the frequency of a frequency-limited test is unknown.
|Specimen||Draw blood in either a plain, red-top tube or a serum gel tube. (GROSSLY HEMOLYZED, OR LIPEMIC SPECIMENS ARE NOT ACCEPTABLE.) Spin down and send 2.0 mL (1.5 mL minimum) of serum refrigerated.
NOTE: THE FOLLOWING SPECIMENS WILL NOT BE TESTED:
1. HEAT-INACTIVATED SPECIMENS
2. CLOUDY SPECIMENS
3. GROSSLY LIPEMIC SPECIMENS
4. HEMOLYZED SPECIMENS
5. OBVIOUS MICROBIAL CONTAMINATION
6. SPECIMENS CONTAINING PARTICULATE MATTER
NOTE: Also draw a lavender-top tube. Spin separate and freeze plasma. Forward along with serum.
|Reference Values||HIVCO: Negative (reported as negative or positive)
HIV-1 RNA quantitative range is 20-10,000,000 copies/mL
|Analytic Time||1 Day (for negatives)|
|Day(s) Test Set Up||Monday through Friday|
86701 if HIV-1 antibody is Detected (if indicated)
86702 if HIV-2 antibody is Detected (if indicated)
87389 HIV 1/2 Antibodies
87536 HIVVL (if indicated)
87806 HIV 1 + HIV 2 AB DIFFERENTIATION (if indicated)