|Test||PROTOPORPHYRINS, FRACTIONATION, RBC (WASHED ERYTHROCYTES|
|Methodology||High-Performance Liquid Chromatography (HPLC) with
|Description||Includes separate quantitation of zinc
protoporphyrins and free protoporphyrins.
See "Porphyria (Acute) Testing Algorithm" and "Porphyria (Cutaneous) Testing Algorithm" in Special Instructions.
Differential diagnosis of chronic intoxication from exposure to a variety of causes including heavy metals and chemicals.
Differential diagnosis in some cases of iron-deficiency anemia.
There are 2 test options: whole blood (#8739 "Protoporphyrins, Fractionation, Whole Blood") and washed RBCs (#31891 "Protoporphyrins, Fractionation, Washed Erythrocytes"). The whole blood option is easiest for clients but requires that the specimen arrive at Mayo Medical Laboratories within 48 hours of draw. When this cannot be ensured, washed RBCs should be submitted.
|Specimen||Submit a full, green-top (heparin) tube of washed erythrocyte suspension (minimum volume, 1 mL of washed and resuspended erythrocytes).
|Reference Values||FREE PROTOPORPHYRIN
<20 µg/dL packed cells
See "The Heme Biosynthetic Pathway" in Special Instructions.
|Analytic Time||2 Days (not reported on Saturday or Sunday)|
|Day(s) Test Set Up||Monday through Friday|