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ACTHH
CORTROSYN STIMULATION TEST (1/2 HOUR)

Test Code ACTHH 
Test CORTROSYN STIMULATION TEST (1/2 HOUR) 
Methodology Electrochemiluminescence Immunoassay (ECLIA) 
Description Useful for the evaluation of adrenal insufficiency in suspected adrenal biosynthetic defect or adrenocorticotropic hormone (ACTH) unresponsiveness.
Test includes two serum cortisol determinations.

No sample should be collected on patients receiving therapy with high biotin doses (i.e. biotin therapy for Multiple Sclerosis or oncology patients; skin, hair and nail supplements, or multivitamins containing > 5 mg/day) until at least 12 hours after the last biotin administration. If unsure, or if the clinical picture does not fit the results please contact the laboratory . We have methods to check for biotin interference as well as for any other interferences and for the accuracy of the results.

Biotin interference would falsely increase the result of this assay. 

Specimen Draw baseline blood in either a plain, red-top tube or serum gel tube. Immediatelly after drawing baseline, clinician administers 0.25 mg ACTH (Cortrosyn). Draw second blood specimen 30 minutes post injection.
Spin down and send 1.0 mL (minimum volume: 0.5 mL) of serum from each specimen, refrigerated. Label tubes with the time drawn. 
Reference Values Cortisol level: > 18 ug/dL before or after the ACTH injection 
Analytic Time 1 day 
Day(s) Test Set Up Monday through Friday 
CPT Code(s) 80400  Cortrosyn Stimulation