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BCRAB
BCR / ABL, TRANSLOCATION 9;22, FLUORESCENCE IN SITU HYBRIDIZATION (FISH)

Test Code BCRAB 
Test BCR / ABL, TRANSLOCATION 9;22, FLUORESCENCE IN SITU HYBRIDIZATION (FISH) 
Methodology Fluorescent In Situ Hybridization (FISH) of Interphase nuclei in white blood cells with probes recognizing regions 9q34 (abl) and 22q11 (bcr). 
Description This test is useful for identifying the "Philadelphia chromosome" (PH chromosome) in patients with chronic myelogenous leukemia (CML), acute lymphocytic leukemia (ALL), or acute myelogenous leukemia (AML) patients. The PH chromosome is caused by the translocation of the 9q34 region into 22q11.

NOTE:
1. This test, when ordered on Medicare patients, is subject to the CMS Medicare Coverage Issues Manual (MCIM) Diagnostic Services policy "Cytogenetic Studies". Please verify that the diagnosis code (ICD-9) you have chosen demonstrates medical necessity for the test as documented in the physician's patient record. The CMS web site or the NorDx Limited Coverage Documentation Guide are available to assist you with this verification. A properly executed Advance Beneficiary Notice (ABN) must be submitted with the specimen if medical necessity is not demonstrated by the ICD-9 code chosen.

2. This test is usually ordered in conjunction with a pathology consultation. The pathology professional component will be billed separately by the consulting pathologist. 

Specimen Submit only 1 of the following specimens:

BLOOD

Draw blood in a green-top (sodium heparin) tube(s), or in a lavender-top (EDTA) tube(s). Send 1.0 mL of sodium heparin or EDTA whole blood at room temperature. Sample must arrive within 24 hours of collection.

BONE MARROW

Place bone marrow in a green-top (sodium heparin) tube(s), or in a lavender-top (EDTA) tube(s). Send 1.0 mL of sodium heparin or EDTA bone marrow at room temperature. Sample must arrive within 24 hours of collection. 

Reference Values Negative (Positive: > 9% of cells contain bcr-abl translocation) 
Analytic Time 2 Days 
Day(s) Test Set Up Monday through Thursday 
CPT Code(s) 88271 x 2  DNA probe, each
88275  Interphase in situ hybridization