|Test||BCR / ABL, TRANSLOCATION 9;22, FLUORESCENCE IN SITU HYBRIDIZATION (FISH)|
|Methodology||Fluorescent In Situ Hybridization (FISH) of Interphase nuclei in white blood cells with probes recognizing regions 9q34 (abl) and 22q11 (bcr).|
|Description||This test is useful for identifying the "Philadelphia chromosome" (PH chromosome) in patients with chronic myelogenous leukemia (CML), acute lymphocytic leukemia (ALL), or acute myelogenous leukemia (AML) patients. The PH chromosome is caused by the translocation of the 9q34 region into 22q11.
2. This test is usually ordered in conjunction with a pathology consultation. The pathology professional component will be billed separately by the consulting pathologist.
|Specimen||Submit only 1 of the following specimens:
Draw blood in a green-top (sodium heparin) tube(s), or in a lavender-top (EDTA) tube(s). Send 1.0 mL of sodium heparin or EDTA whole blood at room temperature. Sample must arrive within 24 hours of collection.
Place bone marrow in a green-top (sodium heparin) tube(s), or in a lavender-top (EDTA) tube(s). Send 1.0 mL of sodium heparin or EDTA bone marrow at room temperature. Sample must arrive within 24 hours of collection.
|Reference Values||Negative (Positive: > 9% of cells contain bcr-abl translocation)|
|Analytic Time||2 Days|
|Day(s) Test Set Up||Monday through Thursday|
88271 x 2 DNA probe, each
88275 Interphase in situ hybridization